Nonculture Prediction of Neisseria meningitidis Susceptibility to Penicillin

Author:

Antignac Aude1,Alonso Jean-Michel1,Taha Muhamed-Kheir1

Affiliation:

1. Unité des Neisseria, Centre National de Référence des Méningocoques, Institut Pasteur, 75724 Paris Cedex 15, France

Abstract

ABSTRACT We developed a nonculture method to predict the susceptibility of Neisseria meningitidis to penicillin G. The penA gene was amplified and submitted to restriction fragment length polymorphism analysis. This approach was first validated with a collection of 75 meningococcal strains of known phenotypes. It was next successfully applied to 29 clinical samples.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference29 articles.

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2. Arreaza L. Vazquez J. A. Proposal for standardization of media and methods to be used on the determination of the susceptibility level of resistance to antibiotics in Neisseria meningitidis Abstracts of the 6th European Monitoring Group on Meningococci 2001 40 43Orebro Sweden.

3. Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA.;Borrow R.;Epidemiol. Infect.,1997

4. siaD PCR ELISA for confirmation and identification of serogroup Y and W135 meningococcal infections.;Borrow R.;FEMS Microbiol. Lett.,1998

5. Interspecies recombination between the penA genes of Neisseria meningitidis and commensal Neisseria species during the emergence of penicillin resistance in N. meningitidis: natural events and laboratory simulation

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