Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates

Author:

Murgier M.1,Belaich J. P.1

Affiliation:

1. Laboratoire de Chimie Bactérienne, C.N.R.S., Marsielle, France

Abstract

A microcalorimetric method using a Tian-Calvet apparatus was utilized for measuring the K m and V max of intact yeast cells for some carbohydrate substrates. These values were obtained for fructose, galactose, and maltose. The heats of ethanolic fermentations were also directly determined, and the results were very close to those obtained by using theoretical calculations and standard values.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference24 articles.

1. Enthalpy changes accompanying the growth of Saccharomyces cerevisiae;Battley E. H.;Physiol. Plant.,1960

2. Thermogenese et croissance de Pseudomonas lindnerien glucose limitant;Belaich J. P.;C. R. Soc. Biol.,1963

3. Microcalorimetric studies on the formation of magnesium complexes of adenine nucleotides;Belaich J. P.;Proc. Nat. Acad. Sci. U.S.A.,1969

4. Influence of aeration and of pantothenate on growth yields of Zymomonas mobilis;Belaich J. P.;J. Bacteriol.,1965

5. Microcalorimetric study of glucose permeation in microbial cells;Belaich J. P.;J. Bacteriol.,1968

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