Structural Analysis of Tobacco Etch Potyvirus HC-Pro Oligomers Involved in Aphid Transmission-Reference-Cited by-同舟云学术

Structural Analysis of Tobacco Etch Potyvirus HC-Pro Oligomers Involved in Aphid Transmission

Published:2005-03-15 Issue:6 Volume:79 Page:3758-3765
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Ruiz-Ferrer Virginia¹,Boskovic Jasminka²,Alfonso Carlos²,Rivas Germán²,Llorca Oscar²,López-Abella Dionisio¹,López-Moya Juan José¹

Affiliation:

1. Departamento de Biología de Plantas

2. Departamento de Estructura y Función de Proteínas, Centro de Investigaciones Biológicas (CIB, CSIC), Ramiro de Maeztu 9, Madrid, Spain

Abstract

ABSTRACT Oligomeric forms of the HC-Pro protein of the tobacco etch potyvirus (TEV) have been analyzed by analytical ultracentrifugation and single-particle electron microscopy combined with three-dimensional (3D) reconstruction. Highly purified HC-Pro protein was obtained from plants infected with TEV by using a modified version of the virus that incorporates a histidine tag at the HC-Pro N terminus (hisHC-Pro). The purified protein retained a high biological activity in solution when tested for aphid transmission. Sedimentation equilibrium showed that the hisHC-Pro preparations were heterogenous in size. Sedimentation velocity confirmed the previous observation and revealed that the active protein solution contained several sedimenting species compatible with dimers, tetramers, hexamers, and octamers of the protein. Electron microscopy fields of purified protein showed particles of different sizes and shapes. The reconstructed 3D structures suggested that the observed particles could correspond to dimeric, tetrameric, and hexameric forms of the protein. A model of the interactions required for oligomerization of the HC-Pro of potyviruses is proposed.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.79.6.3758-3765.2005

Reference53 articles.

1. Ammar, E. D., U. Jarlfors, and T. P. Pirone. 1994. Association of potyvirus helper component protein with virions and the cuticle lining the maxillary food canal and foregut of an aphid vector. Phytopathology84:1054-1060.

2. Anandalakshmi, R., G. J. Pruss, X. Ge, R. Marathe, A. C. Mallory, T. H. Smith, and V. B. Vance. 1998. A viral suppressor of gene silencing in plants. Proc. Natl. Acad. Sci. USA95:13079-13084.

3. Atreya, C. D., and T. P. Pirone. 1993. Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proc. Natl. Acad. Sci. USA90:11919-11923.

4. Atreya, C. D., P. L. Atreya, D. W. Thornbury, and T. P. Pirone. 1992. Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants. Virology191:106-111.

5. Berger, P. H., A. G. Hunt, L. L. Domier, G. M. Hellman, Y. Stram, D. W. Thornbury, and T. P. Pirone. 1989. Expression in transgenic plants of a viral gene product that mediates insect transmission of potyviruses. Proc. Natl. Acad. Sci. USA86:8402-8406.

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