Direct Identification of 80 Percent of Bacteria from Blood Culture Bottles by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a 10-Minute Extraction Protocol-Reference-Cited by-同舟云学术

Direct Identification of 80 Percent of Bacteria from Blood Culture Bottles by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using a 10-Minute Extraction Protocol

Published:2019-02 Issue:2 Volume:57 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Simon Loïc¹^ORCID,Ughetto Estelle¹,Gaudart Alice¹,Degand Nicolas¹,Lotte Romain¹²³,Ruimy Raymond¹²³

Affiliation:

1. Laboratoire de Bactériologie, CHU de Nice, Hôpital de l'Archet 2, Nice, France

2. Université Côte d’Azur, Nice, France

3. INSERM U1065, C3M, Equipe 6 Virulence Microbienne et Signalisation Inflammatoire, Bâtiment Universitaire Archimed, Nice, France

Abstract

Matrix-assisted laser desorption ionization–time of flight mass spectrometry is not widely used to identify bacteria directly from positive blood culture bottles (BCBs) because of overlong protocols. The objective of this work was to develop and evaluate a simple extraction protocol for reliable identification from BCBs.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01278-18

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