Rapid Differentiation of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible Staphylococcus aureus from Blood Cultures by Use of a Direct Cefoxitin Disk Diffusion Test

Author:

Bennett Kelly1,Sharp Susan E.1

Affiliation:

1. Kaiser Permanente, Airport Way Laboratory, 13705 N.E. Airport Way, Portland, Oregon 97230

Abstract

ABSTRACT A total of 276 blood culture bottles with Staphylococcus aureus were tested by direct cefoxitin disk diffusion testing; 105 (38.1%) had zone sizes of ≤17 mm (all 105 had methicillin-resistant S. aureus [MRSA]), 18 (6.5%) had zone sizes that measured 18 mm (17 had MRSA and 1 had methicillin-susceptible S. aureus [MSSA]), 8 (2.9%) had zone sizes that measured 19 mm (6 had MRSA and 2 had MSSA), 8 (2.9%) had zone sizes that measured 20 mm (6 had MRSA and 2 had MSSA), and 137 (49.6%) had zone sizes of ≥21 mm (all 137 had MSSA). Detection of MRSA/MSSA in blood cultures could be reported 10 to 24 h earlier for 88% of cultures with total accuracy.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference22 articles.

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5. Cosgrove, S. E., Y. Qi, K. S. Kaye, S. Harbarth, A. W. Karchmer, and Y. Carmeli. 2005. The impact of methicillin resistance in Staphylococcus aureus bacteremia on patient outcomes: mortality, length of stay, and hospital charges. Infect. Control Hosp. Epidemiol.26:166-174.

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