In Vivo Live Imaging of Oncolytic Mammalian Orthoreovirus Expressing NanoLuc Luciferase in Tumor Xenograft Mice-Reference-Cited by-同舟云学术

In Vivo Live Imaging of Oncolytic Mammalian Orthoreovirus Expressing NanoLuc Luciferase in Tumor Xenograft Mice

Published:2019-07-15 Issue:14 Volume:93 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Kanai Yuta¹,Kawagishi Takahiro¹,Matsuura Yoshiharu²,Kobayashi Takeshi¹

Affiliation:

1. Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

2. Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

Abstract

Engineering of recombinant MRV for improved oncolytic activity has not yet been achieved due to difficulty in generating autonomous replicating MRV harboring transgenes. Here, we constructed a reporter MRV that can be used to monitor cancer-selective infection by oncolytic MRV in a mouse model. Among the numerous oncolytic viruses, MRV has an advantage in that the wild-type virus shows marked oncolytic activity in patients without any notable adverse effects. The reporter MRV developed herein will open avenues to the development of recombinant MRV vectors armed with anticancer transgenes.

Funder

MEXT | Japan Society for the Promotion of Science

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00401-19

Reference46 articles.

1. Integrating oncolytic viruses in combination cancer immunotherapy

2. Publisher Correction: Oncolytic viruses as engineering platforms for combination immunotherapy

3. Reovirus Therapy of Tumors with Activated Ras Pathway