Affiliation:
1. Division of Research, National Jewish Hospital, Denver, Colorado 80206
2. Department of Microbiology, University of Colorado School of Medicine, Denver, Colorado 80220
Abstract
Glycolyzing cells of
Streptococcus faecalis
accumulate K
+
with concurrent extrusion of equivalent amounts of H
+
and Na
+
. An attempt was made to clarify the retionship between the movements of Na
+
and K
+
. Sodium was displaced from cells glycolyzing in the presence of ammonia, diethylamine, tris(hydroxymethyl)aminomethane, and other nitrogenous cations; by contrast, K
+
was completely retained. Accumulation of K
+
by heterologous exchange for Na
+
was not inhibited by antibiotics which facilitate diffusion of K
+
across the membrane, but was blocked by proton conductors. The results indicate that extrusion of Na
+
and H
+
from the cells is a primary, energy-linked process which generates an electrical potential (interior negative); K
+
accumulation occurs in response to this potential. Two mutants deficient in K
+
accumulation and retention were examined in terms of this model. One mutant is apparently defective in exchange of K
+
for H
+
. In the other mutant, exchange of K
+
for Na
+
is impaired.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
76 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献