Screening for Human Immunodeficiency Virus Infection by Use of a Fourth-Generation Antigen/Antibody Assay and Dried Blood Spots: In-Depth Analysis of Sensitivity and Performance Assessment in a Cross-Sectional Study-Reference-Cited by-同舟云学术

Screening for Human Immunodeficiency Virus Infection by Use of a Fourth-Generation Antigen/Antibody Assay and Dried Blood Spots: In-Depth Analysis of Sensitivity and Performance Assessment in a Cross-Sectional Study

Published:2019-12-23 Issue:1 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Stefic Karl¹²^ORCID,Guinard Jerome¹³,Peytavin Gilles⁴,Saboni Leïla⁵,Sommen Cécile⁵,Sauvage Claire⁵,Lot Florence⁵,Laperche Syria⁶,Velter Annie⁵,Barin Francis¹²^ORCID

Affiliation:

1. Centre National de Référence du VIH, Laboratoire Associé, Centre Hospitalier Universitaire Bretonneau, Tours, France

2. Inserm U1259, Université de Tours, Tours, France

3. Laboratoire de Microbiologie, Centre Hospitalier Régional d’Orléans, Orléans, France

4. APHP, Laboratoire de Pharmacologie-Toxicologie et IAME, Inserm UMR 1137, Université Paris 7, Paris, France

5. Santé Publique France, Saint-Maurice, France

6. Département des Agents Transmissibles par le Sang, Centre National de Référence Risques Infectieux Transfusionnels, Institut National de La Transfusion Sanguine, Paris, France

Abstract

We evaluated the performance of a fourth-generation antigen/antibody (Ag/Ab) assay for detecting HIV-1 infection on dried blood spots (DBS) both in a conventional laboratory environment and in an epidemiological survey corresponding to a real-life situation. Although a 2-log loss of sensitivity compared to that with plasma was observed when using DBS in an analytical analysis, the median delay of positivity between DBS and crude serum during the early phase postacute infection was 7 days.

Funder

Agence Nationale de Recherche sur le Sida et les Hepatites Virales

Santé Publique France

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01645-19

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