Rapid SYBR Green I and Modified Probe Real-Time Reverse Transcription-PCR Assays Identify Influenza H1N1 Viruses and Distinguish between Pandemic and Seasonal Strains-Reference-Cited by-同舟云学术

Rapid SYBR Green I and Modified Probe Real-Time Reverse Transcription-PCR Assays Identify Influenza H1N1 Viruses and Distinguish between Pandemic and Seasonal Strains

Published:2009-11 Issue:11 Volume:47 Page:3714-3716
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Yang Ji-Rong¹,Lo Je¹,Liu Jian-Liang¹,Lin Chao-Hua¹,Ho Yu-Lin¹,Chen Chun-Jung¹,Wu Ho-Sheng¹²,Liu Ming-Tsan¹

Affiliation:

1. National Influenza Center, Centers for Disease Control, Taipei, Taiwan, Republic of China

2. School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan, Republic of China

Abstract

ABSTRACT A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid and simultaneous typing and subtyping of influenza viruses.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01646-09

Reference3 articles.

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2. Ward, C. L., M. H. Dempsey, C. J. Ring, R. E. Kempson, L. Zhang, D. Gor, B. W. Snowden, and M. Tisdale. 2004. Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. J. Clin. Virol.29:179-188.

3. CDC protocol of real-time RT-PCR for swine influenza A (H1N1). 2009

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