Real-Time Quantitative Reverse Transcription-PCR Assays Specifically Detecting Bluetongue Virus Serotypes 1, 6, and 8

Author:

Hoffmann Bernd1,Eschbaumer Michael1,Beer Martin1

Affiliation:

1. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riems, Germany

Abstract

ABSTRACT Bluetongue virus (BTV) is a major pathogen of ruminants. Especially serotypes 1, 6, and 8 are of concern to veterinary authorities in central Europe. This article describes highly sensitive real-time reverse transcription-PCR assays directed to BTV genome segment 2 for specific detection of BTV-1, -6, or -8 in animal samples.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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