Spodoptera frugiperda Nuclear Polyhedrosis Virus Genome: Physical Maps for Restriction Endonucleases Bam HI and Hin dIII

Author:

Loh Lambert C.1,Hamm John J.2,Huang Eng-Shang1

Affiliation:

1. Cancer Research Center, University of North Carolina, Chapel Hill, North Carolina 27514

2. U.S. Department of Agriculture, Southeast Area, Southern Grain Insects Research Laboratory, Tifton, Georgia 31793

Abstract

The physical map for the genome of Spodoptera frugiperda nuclear polyhedrosis virus was constructed for restriction endonucleases Bam HI and Hin dIII. The ordering of the restriction fragments was accomplished by cross-blot hybridization of Bam HI, Hin dIII, and Eco RI fragments. The alignment of the Hin dIII fragments within the Bam HI map was achieved by double digestion with the two restriction endonucleases followed by cross-blot hybridization. The results showed that the viral genome consisted of mainly unique sequences. In addition, the circular nature of the viral genome was reaffirmed.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference19 articles.

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2. Molecular weights of Lepidopteran baculovirus DNAs: derivation by electron microscopy;Burgess S.;J. Gen. Virol.,1977

3. Greene P. J. M. C. Bethlach H. M. Goodman and H. W. Boyer. 1974. The EcoRI restriction endonucle ase p. 87-105. In R. B. Wickner (ed.) Methods in molecular biology vol. 7. Marcel Dekker New York.

4. The properties of three Baculoviruses from closely related hosts;Harrap K. A.;Virology,1977

5. A physical map of the DNA regions flanking the rabbit fi-globin gene;Jeffreys A. J.;Cell,1977

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