Indirect hemagglutination test that uses glutaraldehyde-fixed sheep erythrocytes sensitized with extract antigens for detection of Pasteurella antibody

Author:

Sawada T,Rimler R B,Rhoades K R

Abstract

Glutaraldehyde-fixed sheep erythrocytes (GA-SRBC) were used in the indirect hemagglutination test for the detection of Pasteurella antibody. GA-SRBC were stable for at least 6 months. Heat extract or potassium thiocyanate extract antigens of Pasteurella strains could be adsorbed onto GA-SRBC or tanned GA-SRBC, respectively. The indirect hemagglutination test reaction was capsular group specific with heat extract antigen-sensitized GA-SRBC but not potassium thiocyanate extract antigen-sensitized tanned GA-SRBC.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. Serological types of Pasteurella hemolytica;Biberstein E. L.;Cornell Vet.,1960

2. Epidemiological studies on Pasteurella haemolytica in sheep;Biberstein E. L.;J. Comp. Pathol.,1966

3. Hemagglutination with aldehyde-fixed erythrocytes for assay of antigens and antibodies;Bing D. H.;Proc. Soc. Exp. Biol. Med.,1967

4. Studies on Pasteurella multocida. 1. A hemagglutination test for the identification of serological types;Carter G. R.;Am. J. Vet. Res.,1955

5. Formalinized erythrocytes in the haemagglutination test for typing Pasteurella multocida;Carter G. R.;Brit. Vet. J.,1962

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