Author:
Komeno Yukiko,Huang Yi-Jou,Qiu Jinsong,Lin Leo,Xu YiJun,Zhou Yu,Chen Liang,Monterroza Dora D.,Li Hairi,DeKelver Russell C.,Yan Ming,Fu Xiang-Dong,Zhang Dong-Er
Abstract
Myelodysplastic syndromes (MDS) are a group of neoplasms characterized by ineffective myeloid hematopoiesis and various risks for leukemia. SRSF2, a member of the serine/arginine-rich (SR) family of splicing factors, is one of the mutation targets associated with poor survival in patients suffering from myelodysplastic syndromes. Here we report the biological function of SRSF2 in hematopoiesis by using conditional knockout mouse models. Ablation of SRSF2 in the hematopoietic lineage caused embryonic lethality, andSrsf2-deficient fetal liver cells showed significantly enhanced apoptosis and decreased levels of hematopoietic stem/progenitor cells. Induced ablation ofSRSF2in adultMx1-Cre Srsf2flox/floxmice upon poly(I):poly(C) injection demonstrated a significant decrease in lineage−Sca+c-Kit+cells in bone marrow. To reveal the functional impact of myelodysplastic syndromes-associated mutations inSRSF2, we analyzed splicing responses on the MSD-L cell line and found that the missense mutation of proline 95 to histidine (P95H) and a P95-to-R102 in-frame 8-amino-acid deletion caused significant changes in alternative splicing. The affected genes were enriched in cancer development and apoptosis. These findings suggest that intact SRSF2 is essential for the functional integrity of the hematopoietic system and that its mutations likely contribute to development of myelodysplastic syndromes.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
76 articles.
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