Affiliation:
1. Department of Microbiology, Washington State University, Pullman, Washington 99164
Abstract
A negative-selection vector, pHX1, was constructed for use in transposon mutagenesis of
Xenorhabdus nematophilus
ATCC 19061. pHX1 contains the
Bacillus subtilis
levansucrase gene which confers sucrose sensitivity. In addition, various Tn
5
-containing plasmids with different replication origins were transferred by conjugation from
Escherichia coli
into
X. nematophilus
ATCC 19061, and one of these plasmids, pGS9, yields Tn
5
insertion mutants of
X. nematophilus
ATCC 19061. By using these two delivery vehicles, more than 250 putative Tn
5
insertion mutants of
X. nematophilus
ATCC 19061 were isolated and were then characterized. Mutants that were altered in bromothymol blue adsorption, ability to lyse sheep erythrocytes, production of antibiotics on a variety of media, and virulence for
Galleria mellonella
were found.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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