Affiliation:
1. Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, California 90095
Abstract
ABSTRACT
We have identified a novel tRNA methyltransferase in
Saccharomyces cerevisiae
that we designate Trm9. This enzyme, the product of the
YML014w
gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA
Arg3
and 5-methylcarbonylmethyl-2-thiouridine in tRNA
Glu
. In intact yeast cells, disruption of the
TRM9
gene results in the complete loss of these modified wobble bases and increased sensitivity at 37°C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
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