Affinity Purification of Specific Chromatin Segments from Chromosomal Loci in Yeast-Reference-Cited by-同舟云学术

Affinity Purification of Specific Chromatin Segments from Chromosomal Loci in Yeast

Published:2003-12-15 Issue:24 Volume:23 Page:9275-9282
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Griesenbeck Joachim¹,Boeger Hinrich¹,Strattan J. Seth¹,Kornberg Roger D.¹

Affiliation:

1. Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305

Abstract

ABSTRACT Single-copy gene and promoter regions have been excised from yeast chromosomes and have been purified as chromatin by conventional and affinity methods. Promoter regions isolated in transcriptionally repressed and activated states maintain their characteristic chromatin structures. Gel filtration analysis establishes the uniformity of the transcriptionally activated state. Activator proteins interact in the manner anticipated from previous studies in vivo. This work opens the way to the direct study of specific gene regions of eukaryotic chromosomes in diverse functional and structural states.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.23.24.9275-9282.2003

Reference17 articles.

1. Almer, A., H. Rudolph, A. Hinnen, and W. Horz. 1986. Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.EMBO J. 5 : 2689-2696.

2. Ansari, A., T. H. Cheng, and M. R. Gartenberg. 1999 . Isolation of selected chromatin fragments from yeast by site-specific recombination in vivo. Methods 17 : 104-111.

3. Boeger, H., J. Griesenbeck, J. S. Strattan, and R. D. Kornberg. 2003. Nucleosomes unfold completely at a transcriptionally active promoter. Mol. Cell. 11 : 1587-1598.

4. Brazas, R. M., and D. J. Stillman. 1993. The Swi5 zinc-finger and Grf10 homeodomain proteins bind DNA cooperatively at the yeast HO promoter. Proc. Natl. Acad. Sci. USA 90 : 11237-11241.

5. Godde, J. S., and A. P. Wolffe. 1995. Disruption of reconstituted nucleosomes. The effect of particle concentration, MgCl2 and KCl concentration, the histone tails, and temperature. J. Biol. Chem. 270 : 27399-27402.

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