Formation, Location, and Regulation of Endo-1,4-β-Glucanases and β-Glucosidases from Cellulomonas uda

Author:

Stoppok Waltraud1,Rapp Peter1,Wagner Fritz1

Affiliation:

1. Gesellschaft für Biotechnologische Forschung mbH and Lehrstuhl für Biochemie and Biotechnologie der Universität Braunschweig, 2 D-3300 Braunschweig, Federal Republic of Germany

Abstract

The formation and location of endo-1,4-β-glucanases and β-glucosidases were studied in cultures of Cellulomonas uda grown on microcrystalline cellulose, carboxymethyl cellulose, printed newspaper, and some mono- or disaccharides. Endo-1,4-Glucanases were found to be extracellular, but a very small amount of cell-bound endo-1,4-β-glucanase was considered to be the basal endoglucanase level of the cells. The formation of extracellular endo-1,4-β-glucanases was induced by cellobiose and repressed by glucose. Extracellular endoglucanase activity was inhibited by cellobiose but not by glucose. β-Glucosidases, on the other hand, were formed constitutively and found to be cell bound. β-Glucosidase activity was inhibited noncompetitively by glucose. Some characteristics such as the optimal pH for and the thermostability of the endoglucanases and β-glucosidases and the end products of cellulose degradation were determined.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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