Hollow-Fiber Membrane Chamber as a Device for In Situ Environmental Cultivation-Reference-Cited by-同舟云学术

Hollow-Fiber Membrane Chamber as a Device for In Situ Environmental Cultivation

Published:2009-06 Issue:11 Volume:75 Page:3826-3833
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Aoi Yoshiteru¹,Kinoshita Tomoyuki²,Hata Toru²,Ohta Hiroaki³,Obokata Haruko³,Tsuneda Satoshi³

Affiliation:

1. Waseda Institute for Advanced Study, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan

2. Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan

3. Department of Life Science and Medical Bio-Science, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan

Abstract

ABSTRACT A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02542-08

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4. Effect of Signal Compounds and Incubation Conditions on the Culturability of Freshwater Bacterioplankton

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