Author:
Mieschendahl M,Müller-Hill B
Abstract
We describe the construction and properties of an F' factor which carries the temperature-sensitive cI857 allele of the repressor gene of coliphage lambda and which lacks the lambda cro function. This episome can easily be transferred to any F- and F' Escherichia coli strain, thus facilitating the construction and regulation of lambda promoter-dependent expression systems without the use of defective prophages.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference25 articles.
1. Construction of plasmid cloning vehicles that promote gene expression from bacteriophage lambda PL promoter;Bernard H.;Gene,1979
2. Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators;Brosius J.;Gene,1984
3. Calef E. A. Avitabile L. Del Giudice C. Marchelli T. Menna Z. Neubauer and A. Soller. 1971. The genetics of the antiimmune phenotype of defective A Iysogens p. 609-620. In A. D. Hershey (ed.) The bacteriophage A. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
4. High expression of cloned genes in E. coli and its consequences;Carrier M. J.;Trends Biotechnol.,1983
5. Quantitative aspects of gene expression in a X-trp fusion operon;Davison J.;Mol. Gen. Genet.,1974
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