Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration-Reference-Cited by-同舟云学术

Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Terrones-Fernandez I.¹²,Casino P.¹,López A.¹,Peiró S.¹,Ríos S.³⁴,Nardi-Ricart A.⁵,García-Montoya E.⁵⁶,Asensio D.¹,Marqués A. M.⁷,Castilla R.²,Gamez-Montero P. J.²,Piqué N.⁷⁸^ORCID

Affiliation:

1. Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Barcelona, Catalonia, Spain

2. CATMech. Department of Fluid Mechanics, Universitat Politecnica de Catalunya, Terrassa, Catalonia, Spain

3. Department of Genetics, Microbiology and Statistics, Biology Faculty, Universitat de Barcelona, Barcelona, Catalonia, Spain

4. Department of Biochemistry and Biotechnology, Human Nutrition Unit, Universitat Rovira i Virgili, Reus, Catalonia, Spain

5. Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Catalonia, Spain

6. Pharmacotherapy, Pharmacogenetics and Pharmaceutical Technology Research Group, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Catalonia, Spain

7. Microbiology Section, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona (UB), Barcelona, Catalonia, Spain

8. Institut de Recerca en Nutrició i Seguretat Alimentària de la UB (INSA-UB), Universitat de Barcelona, Barcelona, Catalonia, Spain

Abstract

In the era of rapid microbiological methods, there is a need to improve long-established culture techniques. Drawbacks of the pour plate method include having to melt each medium separately before seeding.

Funder

Doctorats Industrials Generalitat de Catalunya

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03161-22

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