Comprehensive, Comparative Evaluation of 35 Manual SARS-CoV-2 Serological Assays

Author:

Dimech Wayne1ORCID,Curley Shannon1,Subissi Lorenzo2,Ströher Ute2,Perkins Mark D.2,Cunningham Jane2

Affiliation:

1. National Serology Reference Laboratory, Fitzroy, Australia

2. World Health Organization, Geneva, Switzerland

Abstract

The markets have been flooded with hundreds of SARS-CoV-2 serology tests, and although there are many published reports on their performance, comparative reports are far fewer and tend to be limited to only a few tests. In this report, we comparatively assessed 35 rapid diagnostic tests or microtiter plate enzyme immunoassays (EIAs) using a large set of samples from individuals with a history of mild to moderate COVID-19, commensurate with the target population for serosurveillance, which included serum samples from individuals previously infected, at undetermined time periods, with other seasonal human coronaviruses, Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-1.

Funder

World Health Organization

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Reference21 articles.

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4. World Health Organization. 2020. Advice on the use of point-of-care immunodiagnostic tests for COVID-19. World Health Organization, Geneva, Switzerland.

5. World Health Organization. 2020. Expression of interest—general information and timelines. World Health Organization, Geneva, Switzerland. https://www.who.int/docs/default-source/coronaviruse/eoi_general-info_timelines.pdf?sfvrsn=215a8b28_5. Accessed 30 May 2022.

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