Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases-Reference-Cited by-同舟云学术

Electrostatic Interaction with the Bacterial Cell Envelope Tunes the Lytic Activity of Two Novel Peptidoglycan Hydrolases

Published:2022-06-29 Issue:3 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Wysocka Alicja¹^ORCID,Łężniak Łukasz¹,Jagielska Elżbieta¹²^ORCID,Sabała Izabela¹²^ORCID

Affiliation:

1. International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland

2. Mossakowski Medical Research Institute Polish Academy of Sciences, Warsaw, Poland

Abstract

This study shows direct relationship between the surface charge of two recently described enzymes, SpM23_A and SpM23_B, and bacterial cell walls. We demonstrate that by (i) surface charge probing of bacterial strains collection, (ii) reduction of the net charge of the positively charged enzyme, and (iii) altering the net charge of the bacterial surface by modifying the content and composition of teichoic acids.

Funder

Fundacja na rzecz Nauki Polskiej

Narodowe Centrum Nauki

Narodowa Agencja Wymiany Akademickiej

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00455-22

Reference75 articles.

1. Different walls for rods and balls: the diversity of peptidoglycan