Unveiling the super tolerance of
Candida nivariensis
to oxidative stress: insights into the involvement of a catalase
-
Published:2024-01-11
Issue:
Volume:
Page:
-
ISSN:2165-0497
-
Container-title:Microbiology Spectrum
-
language:en
-
Short-container-title:Microbiol Spectr
Author:
Qi Yanhua1ORCID,
Qin Qijian1,
Liao Guiyan1,
Tong Lige1,
Jin Cheng2ORCID,
Wang Bin1ORCID,
Fang Wenxia1ORCID
Affiliation:
1. Institute of Biological Science and Technology, Guangxi Academy of Sciences, Nanning, Guangxi, China
2. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Abstract
ABSTRACT
Yeast cells involved in fermentation processes face various stressors that disrupt redox homeostasis and cause cellular damage, making the study of oxidative stress mechanisms crucial. In this investigation, we isolated a resilient yeast strain,
Candida nivariensis
GXAS-CN, capable of thriving in the presence of high concentrations of H
2
O
2
. Transcriptomic analysis revealed the up-regulation of multiple antioxidant genes in response to oxidative stress. Deletion of the catalase gene
Cncat
significantly impacted H
2
O
2
-induced oxidative stress. Enzymatic analysis of recombinant
Cn
Cat highlighted its highly efficient catalase activity and its essential role in mitigating H
2
O
2
. Furthermore, over-expression of
Cn
Cat in
Saccharomyces cerevisiae
improved oxidative resistance by reducing intracellular ROS accumulation. The presence of multiple stress-responsive transcription factor binding sites at the promoters of antioxidative genes indicates their regulation by different transcription factors. These findings demonstrate the potential of utilizing the remarkably tolerant
C. nivariensis
GXAS-CN or enhancing the resistance of
S. cerevisiae
to improve the efficiency and cost-effectiveness of industrial fermentation processes.
IMPORTANCE
Enduring oxidative stress is a crucial trait for fermentation strains. The importance of this research is its capacity to advance industrial fermentation processes. Through an in-depth examination of the mechanisms behind the remarkable H
2
O
2
resistance in
Candida nivariensis
GXAS-CN and the successful genetic manipulation of this strain, we open the door to harnessing the potential of the catalase
Cn
Cat for enhancing the oxidative stress resistance and performance of yeast strains. This pioneering achievement creates avenues for fine-tuning yeast strains for precise industrial applications, ultimately leading to more efficient and cost-effective biotechnological processes.
Funder
Guangxi Major Science and Technology Innovation Base
Guangxi Science and Technology Base and Talent Special Project
广西壮族自治区科学技术厅 | Natural Science Foundation of Guangxi Zhuang Autonomous Region
Research Start-up Funding of Guangxi Academy of Science
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology