Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile Process to Perform Single-Molecule Localization Microscopy in Mycoplasmas

Author:

Rideau Fabien1,Villa Audrey1,Belzanne Pauline2,Verdier Emeline2,Hosy Eric2,Arfi Yonathan1ORCID

Affiliation:

1. Université de Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, Villenave d’Ornon, France

2. Interdisciplinary Institute for Neuroscience, CNRS, Université de Bordeaux, IINS, UMR 5297, Bordeaux, France

Abstract

Mycoplasmas are important models in biology, as well as highly problematic pathogens in the medical and veterinary fields. The very small sizes of these bacteria, well below a micron, limits the usefulness of traditional fluorescence imaging methods, as their resolution limit is similar to the dimensions of the cells.

Funder

Agence Nationale de la Recherche

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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