A Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Direct-from-Urine-Specimen Diagnostic for Gram-Negative Pathogens-Reference-Cited by-同舟云学术

A Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Direct-from-Urine-Specimen Diagnostic for Gram-Negative Pathogens

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Yang Hyojik¹,Smith Richard D.¹²^ORCID,Sumner Kylie P.¹³,Goodlett David R.³⁴,Johnson J. Kristie²,Ernst Robert K.¹^ORCID

Affiliation:

1. Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, Maryland, USA

2. Department of Pathology, School of Medicine, University of Maryland, Baltimore, Maryland, USA

3. Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada

4. International Centre for Cancer Vaccine Science, University of Gdansk, Gdansk, Poland

Abstract

This study describes and evaluates a direct-from-urine extraction method that allows identification of Gram-negative bacteria via MALDI-TOF MS within 1 h. Currently, identification of urinary pathogens requires 24 h of culture prior to identification.

Funder

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03730-22

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