A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum-Reference-Cited by-同舟云学术

A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum

Published:2022-06-29 Issue:3 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Liang Xiaoying¹,Boonhok Rachasak¹,Siddiqui Faiza Amber¹,Xiao Bo²,Li Xiaolian¹,Qin Junling¹,Min Hui¹,Jiang Lubin²,Cui Liwang¹³^ORCID,Miao Jun¹³^ORCID

Affiliation:

1. Department of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA

2. Unit of Human Parasite Molecular and Cell Biology, Key Laboratory of Molecular Virology and Immunology, Pasteur Institute of Shanghai, Chinese Academy of Sciences, Shanghai, People’s Republic of China

3. Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, Florida, USA

Abstract

Understanding the fundamental biology of malaria parasites through functional genetic/genomic studies is critical for identifying novel targets for antimalarial development. Conditional knockout/knockdown systems are required to study essential genes in the haploid blood stages of the parasite.

Funder

HHS | National Institutes of Health

The startup fund from Morsani College of Medicine

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02782-21

Reference75 articles.

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3. Generating conditional gene knockouts in Plasmodium – a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9

4. A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum

5. Robust inducible Cre recombinase activity in the human malaria parasite P lasmodium falciparum enables efficient gene deletion within a single asexual erythrocytic growth cycle

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