A Partially Multiplexed HIV Drug Resistance (HIVDR) Assay for Monitoring HIVDR Mutations of the Protease, Reverse-Transcriptase (PRRT), and Integrase (INT)-Reference-Cited by-同舟云学术

A Partially Multiplexed HIV Drug Resistance (HIVDR) Assay for Monitoring HIVDR Mutations of the Protease, Reverse-Transcriptase (PRRT), and Integrase (INT)

Published:2022-06-29 Issue:3 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

DeVos Joshua¹^ORCID,McCarthy Kimberly²,Sewe Victor³,Akinyi Grace³,Junghae Muthoni²,Opollo Valarie³,Nouhin Janin⁴,Shafer Robert⁴,Zeh Clement¹,Ramos Artur¹,Alexander Heather¹,Chang Joy¹

Affiliation:

1. International Laboratory Branch, Division of Global HIV and Tuberculosis (TB), Center for Global Health, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA

2. CDC Kenya, Nairobi, Kenya

3. HIV Research Laboratory, Kenya Medical Research Institute-Center for Global Health Research, Kisumu, Kenya

4. Stanford University, Stanford, California, USA

Abstract

This HIVDR genotyping assay works for both plasma and DBS samples, requires low sample input, and is sensitive. This assay has the potential to be a user-friendly and cost-effective HIVDR assay because of its partially multiplexed design. Application of this genotyping assay will help HIVDR monitoring in HIV high-burdened countries using a DGT-based HIV drug regimen recommended by the U.S. President's Emergency Plan for AIDS Relief and the WHO.

Funder

U.S. President's Emergency Plan for AIDS Relief

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01776-21

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