Developing an Amplification Refractory Mutation System-Quantitative Reverse Transcription-PCR Assay for Rapid and Sensitive Screening of SARS-CoV-2 Variants of Concern-Reference-Cited by-同舟云学术

Developing an Amplification Refractory Mutation System-Quantitative Reverse Transcription-PCR Assay for Rapid and Sensitive Screening of SARS-CoV-2 Variants of Concern

Published:2022-02-23 Issue:1 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Xiong Dongyan¹²,Zhang Xiaoxu¹,Shi Mengjuan¹²,Wang Nuo¹,He Ping¹²,Dong Zhuo³,Zhong Jie³,Luo Jing³,Wang Yong³,Yu Junping¹²,Wei Hongping¹²^ORCID

Affiliation:

1. CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China

2. University of Chinese Academy of Sciences, Beijing, China

3. Hubei International Travel Healthcare Center, Wuhan Customs Port Outpatient Department, Wuhan, China

Abstract

The current stage of the pandemic, led by SARS-CoV-2 variants of concern (VOCs), underscores the necessity to develop a cost-effective and rapid molecular diagnosis assay to differentiate the VOCs. In this study, over 1 million SARS-CoV-2 genomic sequences of high quality from GISAID were analyzed and a network of the common mutations of the lineages was constructed.

Funder

Urgent project for COVID-19 control by Hubei province

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01438-21

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