Evaluation of Host Depletion and Extraction Methods for Shotgun Metagenomic Analysis of Bovine Vaginal Samples-Reference-Cited by-同舟云学术

Evaluation of Host Depletion and Extraction Methods for Shotgun Metagenomic Analysis of Bovine Vaginal Samples

Published:2022-04-27 Issue:2 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Ong Chian Teng¹^ORCID,Boe-Hansen Gry²,Ross Elizabeth M.¹,Blackall Patrick J.¹,Turni Conny¹,Hayes Ben J.¹,Tabor Ala E.¹³

Affiliation:

1. Queensland Alliance for Agriculture and Food Innovation, Centre for Animal Science, The University of Queensland, Brisbane, Queensland, Australia

2. School of Veterinary Science, Faculty of Science, The University of Queensland, Brisbane, Queensland, Australia

3. School of Chemistry and Molecular Bioscience, Faculty of Science, The University of Queensland, Brisbane, Queensland, Australia

Abstract

In addition to the host tissue collected during the sampling process, bovine vaginal samples are saturated with large amounts of extracellular DNA and secreted proteins that are essential for physiological purposes, including the reproductive cycle and immune defense. Due to the high host-to-microbe genome ratio, which hampers the sequencing efficacy for metagenome samples and the recovery of the actual metagenomic profiles, bovine vaginal samples cannot benefit from the full potential of shotgun sequencing.

Funder

Meat and Livestock Australia

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00412-21

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