Comparison of Eleven RNA Extraction Methods for Poliovirus Direct Molecular Detection in Stool Samples-Reference-Cited by-同舟云学术

Comparison of Eleven RNA Extraction Methods for Poliovirus Direct Molecular Detection in Stool Samples

Published:2023-04-13 Issue:2 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Akello Joyce Odeke¹^ORCID,Bujaki Erika²,Shaw Alexander G.¹^ORCID,Khurshid Adnan³,Arshad Yasir³,Troman Catherine¹,Majumdar Manasi²,O'Toole Áine⁴,Rambaut Andrew⁴,Alam Muhammad Masroor³,Martin Javier²^ORCID,Grassly Nicholas C.¹

Affiliation:

1. Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom

2. Division of Vaccines, National Institute for Biological Standards and Control (NIBSC), MHRA, Potters Bar, United Kingdom

3. Department of Virology, National Institute for Health, Islamabad, Pakistan

4. Institute of Evolutionary Biology, University of Edinburgh, Ashworth Laboratories, Edinburgh, United Kingdom

Abstract

Poliovirus, the causative agent of poliomyelitis, is a target of global eradication led by the World Health Organization since 1988. Direct molecular detection and genomic sequencing without virus propagation in cell culture is arguably a critical tool in the final stages of polio eradication.

Funder

Bill and Melinda Gates Foundation

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.04252-22

Reference27 articles.

1. Paralytic poliomyelitis: seasoned strategies, disappearing disease

2. World Health Organisation. 2003. Guidelines for environmental surveillance of poliovirus circulation. https://polioeradication.org/wp-content/uploads/2016/07/WHO_V-B_03.03_eng.pdf. Accessed 19 August 2022.

3. World Health Organisation. 2015. WHO Global Action Plan to minimize poliovirus facility-associated risk after type-specific eradication of wild polioviruses and sequential cessation of oral polio vaccine use. https://apps.who.int/iris/handle/10665/208872. Accessed 1 July 2022.

4. Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network

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