Comparative Assessment of Filtration- and Precipitation-Based Methods for the Concentration of SARS-CoV-2 and Other Viruses from Wastewater

Author:

Farkas Kata12ORCID,Pellett Cameron1,Alex-Sanders Natasha1,Bridgman Matthew T. P.1,Corbishley Alexander3ORCID,Grimsley Jasmine M. S.4,Kasprzyk-Hordern Barbara5,Kevill Jessica L.1,Pântea Igor1,Richardson-O’Neill India S.1,Lambert-Slosarska Kathryn1,Woodhall Nick1,Jones Davey L.16

Affiliation:

1. Centre for Environmental Biotechnology, School of Natural Sciences, Bangor University, Bangor, Gwynedd, United Kingdom

2. School of Ocean Sciences, Bangor University, Anglesey, United Kingdom

3. Royal (Dick) School of Veterinary Studies and The Roslin Institute, University of Edinburgh, Roslin, United Kingdom

4. UK Health Security Agency, Environmental Monitoring for Health Protection, London, United Kingdom

5. Department of Chemistry, University of Bath, Bath, United Kingdom

6. Food Futures Institute, Murdoch University, Murdoch, Western Australia, Australia

Abstract

As wastewater-based epidemiology is utilized for the surveillance of COVID-19 at the community level in many countries, it is crucial to develop and validate reliable methods for virus detection in sewage. The most important step in viral detection is the efficient concentration of the virus particles and/or their genome for subsequent analysis.

Funder

Department of Health, Social Services and Public Safety, UK Government

EC | European Regional Development Fund

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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