Detection and Stability of SARS-CoV-2 in Three Self-Collected Specimen Types: Flocked Midturbinate Swab (MTS) in Viral Transport Media, Foam MTS, and Saliva-Reference-Cited by-同舟云学术

Detection and Stability of SARS-CoV-2 in Three Self-Collected Specimen Types: Flocked Midturbinate Swab (MTS) in Viral Transport Media, Foam MTS, and Saliva

Published:2022-06-29 Issue:3 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Veguilla Vic¹^ORCID,Fowlkes Ashley L.¹,Bissonnette Adam²,Beitel Shawn³,Gaglani Manjusha⁴⁵,Porucznik Christina A.⁶^ORCID,Stockwell Melissa S.⁷⁸,Tyner Harmony L.⁹,Naleway Allison L.¹⁰,Yoon Sarang K.⁶,Caban-Martinez Alberto J.¹¹,Wesley Meredith G.¹²,Duque Jazmin¹²,Jeddy Zuha¹²,Stanford Joseph B.⁶^ORCID,Daugherty Michael¹,Dixon Ashton¹,Burgess Jefferey L.³,Odean Marilyn⁹¹³,Groom Holly C.¹⁰,Phillips Andrew L.⁶,Schaefer-Solle Natasha¹¹,Mistry Peenaz¹²,Rolfes Melissa A.¹,Thompson Mark¹,Dawood Fatimah S.¹,Meece Jennifer²

Affiliation:

1. Centers for Disease Control and Prevention, Atlanta, Georgia, USA

2. Integrated Research & Development Laboratory, Marshfield Clinic Research Institute, Marshfield, Wisconsin, USA

3. Mel and Enid Zuckerman College of Public Health, University of Arizona, Tucson, Arizona, USA

4. Baylor Scott and White Health, Temple, Texas, USA

5. Texas A&M University College of Medicine, Temple, Texas, USA

6. Division of Public Health, Department of Family and Preventive Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA

7. Division of Child and Adolescent Health, Department of Pediatrics, Columbia University Irving Medical Center, New York, New York, USA

8. Department of Population and Family Health, Mailman School of Public Health, Columbia University Irving Medical Center, New York, New York, USA

9. St. Luke’s Regional Health Care System, Duluth, Minnesota, USA

10. Kaiser Permanente Northwest Center for Health Research, Portland, Oregon, USA

11. Leonard M. Miller School of Medicine, University of Miami, Miami, Florida, USA

12. Abt Associates, Inc., Cambridge, Massachusetts, USA

13. The Whiteside Institute for Clinical Research, Duluth, Minnesota, USA

Abstract

Findings from this analysis indicate that (1) self-collection of flocked and foam MTS and saliva samples is feasible in both adults and children, (2) foam MTS with VTM and saliva are both viable and reasonable alternatives to traditional flocked MTS in VTM for SARS-CoV-2 detection, and (3) these sample types may be stored and transported at ambient temperatures for up to 7 days without compromising sample quality. These findings support methods of sample collection for SARS-CoV-2 detection that may facilitate widespread community testing in the setting of supply and personnel shortages during the current pandemic.

Funder

HHS | Centers for Disease Control and Prevention

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01033-22

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