Evaluation of a Novel Commercial Real-Time PCR Assay for the Simultaneous Detection of <i>Cryptosporidium</i> spp., Giardia duodenalis, and Entamoeba histolytica-Reference-Cited by-同舟云学术

Evaluation of a Novel Commercial Real-Time PCR Assay for the Simultaneous Detection of Cryptosporidium spp., Giardia duodenalis, and Entamoeba histolytica

Published:2022-06-29 Issue:3 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Dashti Alejandro¹^ORCID,Alonso Henar²,Escolar-Miñana Cristina³,Köster Pamela C.¹^ORCID,Bailo Begoña¹^ORCID,Carmena David¹⁴^ORCID,González-Barrio David¹^ORCID

Affiliation:

1. Parasitology Reference and Research Laboratory, National Centre for Microbiology, Carlos III Health Institute, Madrid, Spain

2. Department of Microbiology, Paediatrics, Radiology, and Public Health, Faculty of Medicine, University of Zaragoza, Saragossa, Spain

3. Department of Animal Production and Food Science, Faculty of Veterinary, University of Zaragoza, Saragossa, Spain

4. Centro de Investigación Biomédica en Red de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain

Abstract

Thorough independent assessment of the diagnostic performance of novel diagnostic assays is essential to ascertain their true usefulness and applicability in routine clinical practice. This is particularly true for commercially available kits based on multiplex real-time PCR aimed to detect and differentiate multiple pathogens in a single biological sample.

Funder

MEC | Instituto de Salud Carlos III

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00531-22

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