Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens-Reference-Cited by-同舟云学术

Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Eamsakulrat Pruke¹,Santanirand Pitak²,Phuphuakrat Angsana¹^ORCID

Affiliation:

1. Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

2. Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

Abstract

16S rRNA gene sequencing has been increasingly used in clinical practice. Using the final diagnosis as a gold standard, the sensitivity of 16S rRNA gene sequencing was fair. In the setting with no 16S rRNA gene sequencing test ordering restrictions, only small percentages of the test results had an impact on antimicrobial management. Restrictions on test requests should be developed to maximize the benefit of the test.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02094-22

Reference32 articles.

1. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

2. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists

3. CLSI. 2017. Methods for the identification of cultured microorganisms using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, vol 1. Clinical and Laboratory Standards Institute, Wayne, PA.

4. 16S rRNA sequencing in routine bacterial identification: A 30-month experiment

5. CLSI. 2018. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing, vol 2. Clinical and Laboratory Standards Institute, Wayne, PA.