Development of a Novel Enzyme-Linked Immunosorbent Assay and Lateral Flow Test System for Improved Serodiagnosis of Visceral Leishmaniasis in Different Areas of Endemicity-Reference-Cited by-同舟云学术

Development of a Novel Enzyme-Linked Immunosorbent Assay and Lateral Flow Test System for Improved Serodiagnosis of Visceral Leishmaniasis in Different Areas of Endemicity

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Mahdavi Rouzbeh¹,Shams-Eldin Hosam¹,Witt Sandra¹,Latz Andreas²,Heinz Daniela²,Fresco-Taboada Alba³,Aira Cristina³,Hübner Marc P.⁴⁵,Sukyte Dalia¹,Visekruna Alexander¹,Teixeira Henrique C.⁶,Abass Elfadil⁷^ORCID,Steinhoff Ulrich¹^ORCID

Affiliation:

1. Institute of Medical Microbiology and Hospital Hygiene, Philipps University Marburg, Marburg, Germany

2. Novatec Immundiagnostica GmbH, part of Gold Standard Diagnostics Europe, Dietzenbach, Germany

3. Eurofins-Immunologia y Genética Aplicada S.A. (Eurofins Ingenasa S.A.), Madrid, Spain

4. Institute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany

5. German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany

6. Department of Parasitology, Microbiology and Immunology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil

7. Department of Clinical Laboratory Science, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia

Abstract

Reliable and field suitable serodiagnosis of visceral leishmaniasis (VL) in East Africa has until now been a big challenge due to low sensitivity and cross-reactivity with other pathogens. To improve VL serodiagnosis, a new recombinant kinesin antigen from Leishmania infantum (rKLi8.3) was developed and tested with a panel of sera from Sudanese, Indian, and South American patients diagnosed with VL or other infectious diseases.

Funder

Hessian Excellence Cluster, Loewe Center, Druid

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.04338-22

Reference32 articles.

1. Research priorities for Chagas disease, human African trypanosomiasis and leishmaniasis: technical report of the TDR Disease Reference Group on Chagas Disease, Human African Trypanosomiasis and Leishmaniasis Introduction;WHO Technical Report Series;World Health Organ Tech Rep Ser,2012

2. rKLO8, a Novel Leishmania donovani – Derived Recombinant Immunodominant Protein for Sensitive Detection of Visceral Leishmaniasis in Sudan

3. Bern C. 2021. Visceral leishmaniasis: clinical manifastation and diagnosis. https://www.uptodate.com/contents/visceral-leishmaniasis-clinical-manifestations-and-diagnosis.

4. Leishmaniasis

5. Improvement of a direct agglutination test for field studies of visceral leishmaniasis

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