A <i>pyrF</i> -Based Efficient Genetic Manipulation Platform in Acinetobacter baumannii To Explore the Vital DNA Components of Adaptive Immunity for I-F CRISPR-Cas-Reference-Cited by-同舟云学术

A pyrF -Based Efficient Genetic Manipulation Platform in Acinetobacter baumannii To Explore the Vital DNA Components of Adaptive Immunity for I-F CRISPR-Cas

Published:2022-10-26 Issue:5 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Wu Shuqi¹,Xu Run¹,Su Mengjiao¹,Gao Can¹²,Liu Yang¹,Chen Yujia¹,Luan Guangxin¹^ORCID,Jia Xu¹,Wang Rui¹³^ORCID

Affiliation:

1. Non-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical College, Chengdu, Sichuan, China

2. Yan’an Key Laboratory of Microbial Drug Innovation and Transformation, School of Basic Medicine, Yan’an University, Yan’an, Shaanxi, China

3. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

Abstract

In this study, we developed the widely applicable and efficient pyrF -based selection and counterselection system in A. baumannii for gene manipulation. In most cases, this pyrF /5-FOA genetic manipulation system was very effective and enabled us to obtain marker-free mutants in a very short period of time.

Funder

National Natural Science Foundation of China

Chengdu Medical College

The Research Fund of Non-coding RNA and Drug Discovery Key Laboratory of Sichuan Province

College Students’ Innovative Entrepreneurial Training Plan Program

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01957-22

Reference54 articles.