Simple and Economical Extraction of Viral RNA and Storage at Ambient Temperature-Reference-Cited by-同舟云学术

Simple and Economical Extraction of Viral RNA and Storage at Ambient Temperature

Published:2022-06-29 Issue:3 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Hernandez Sarah¹^ORCID,Cardozo Fátima²,Myers David R.³⁴,Rojas Alejandra⁵,Waggoner Jesse J.¹⁶^ORCID

Affiliation:

1. Emory University, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA

2. Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, Departamento de Salud Pública, San Lorenzo, Paraguay

3. Wallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia, USA

4. Aflac Cancer and Blood Disorders Center of Children’s Healthcare of Atlanta, Department of Pediatrics, Emory University, Atlanta, Georgia, USA

5. Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, Departamento de Producción, San Lorenzo, Paraguay

6. Rollins School of Public Health, Department of Global Health, Atlanta, Georgia, USA

Abstract

RNA extraction methods and ultra-cold storage requirements limit molecular testing for common viruses. We developed a simple, flexible, and economical method that simultaneously addresses these limitations.

Funder

Doris Duke Charitable Foundation

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00859-22

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