Evaluation of Four Strategies for SARS-CoV-2 Detection: Characteristics and Prospects-Reference-Cited by-同舟云学术

Evaluation of Four Strategies for SARS-CoV-2 Detection: Characteristics and Prospects

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Chen Yuqing¹²³^ORCID,Ma Yu¹²³,Han Yanxi¹³,Diao Zhenli¹²³,Chang Lu¹²³,Li Jinming¹²³,Zhang Rui¹²³

Affiliation:

1. National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, Beijing, People’s Republic of China

2. Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China

3. Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, People’s Republic of China

Abstract

In the study, we reported an evaluation of 4 detection strategies implemented in different scenarios for SARS-CoV-2 detection: single sampling tests, 20-in-1 pooling tests, nucleic acid point-of-care tests, and rapid antigen tests. 3 SARS-CoV-2-inactivated SARS-CoV-2 cell culture supernatants and 5 recombinant SARS-CoV-2 nucleocapsid proteins were used for evaluation. In this analysis, we found that for the WT, Delta, and Omicron supernatants, the lowest concentrations to achieve 100% detection rates of single sampling tests ranged between 1.28 × 10 2 to 1.02 × 10 3 , 1.28 × 10 2 to 4.10 × 10 3 , and 1.28 × 10 2 to 2.05 × 10 3 copies/mL.

Funder

MOST | National Key Research and Development Program of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02143-22

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