Red Blood Cell Lysis Pretreatment Can Significantly Improve the Yield of Treponema pallidum DNA from Blood-Reference-Cited by-同舟云学术

Red Blood Cell Lysis Pretreatment Can Significantly Improve the Yield of Treponema pallidum DNA from Blood

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Wei Zi-Han¹,Xie Lin¹,Wang Yong-Jing¹,Zhuang Jiang-Xing²,Niu Jian-Jun¹³,Liu Li-Li¹³⁴^ORCID

Affiliation:

1. Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China

2. Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, School of Medicine, Xiamen University, Xiamen, China

3. Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China

4. Xiamen Clinical Laboratory Quality Control Center, Xiamen, China

Abstract

Syphilis is a sexually transmitted disease caused by T. pallidum that can spread into the blood. T. pallidum DNA can be detected in blood by PCR but with low sensitivity. Few studies have applied RBC lysis pretreatment to blood T. pallidum DNA extraction.

Funder

National Natural Science Founation of China

The Key projects of the Natural Science Foundation of Fujian Province

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.05198-22

Reference21 articles.

1. Sexually acquired syphilis

2. Timing of primary syphilis treatment and impact on the development of treponemal antibodies: a cross-sectional clinic-based study

3. The Clinical Characteristics and Serological Outcomes of Infants With Confirmed or Suspected Congenital Syphilis in Shanghai, China: A Hospital-Based Study

4. Unusual clinical manifestation and challenging serological interpretation of syphilis: insights from a case report

5. Evaluation of a PCR Test for Detection of Treponema pallidum in Swabs and Blood

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Elevated mtDNA content in RBCs promotes oxidative stress may be responsible for faster senescence in men;Archives of Gerontology and Geriatrics;2024-10

2. Platelet-derived major histocompatibility complex class I coating on Treponema pallidum attenuates natural killer cell lethality;Virulence;2024-05-14

3. Neurosyphilis: insights into its pathogenesis, susceptibility, diagnosis, treatment, and prevention;Frontiers in Neurology;2024-01-11