Prospective Study of the Detection of Bacterial Pathogens in Pediatric Clinical Specimens Using the Melting Temperature Mapping Method-Reference-Cited by-同舟云学术

Prospective Study of the Detection of Bacterial Pathogens in Pediatric Clinical Specimens Using the Melting Temperature Mapping Method

Published:2022-06-29 Issue:3 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Uejima Yoji¹²^ORCID,Suganuma Eisuke¹,Ohnishi Takuma¹,Takei Haruka¹,Furuichi Mihoko¹,Sato Satoshi¹,Kawano Yutaka¹,Kitajima Isao²,Niimi Hideki²

Affiliation:

1. Division of Infectious Diseases and Immunology, Saitama Children’s Medical Center, Saitama, Japan

2. Department of Clinical Laboratory and Molecular Pathology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan

Abstract

This study provides novel insights regarding the use of the melting temperature ( T m ) mapping method to identify the dominant bacteria in samples collected from pediatric patients. We designed a new set of primers for commercial use and developed a database of different bacteria that can be identified using these primers.

Funder

Japan Agency for Medical Research and Development

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00198-22

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