Sensitive Detection of Asymptomatic and Symptomatic Malaria with Seven Novel Parasite-Specific LAMP Assays and Translation for Use at Point-of-Care-Reference-Cited by-同舟云学术

Sensitive Detection of Asymptomatic and Symptomatic Malaria with Seven Novel Parasite-Specific LAMP Assays and Translation for Use at Point-of-Care

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Malpartida-Cardenas Kenny¹²^ORCID,Moser Nicolas²,Ansah Felix³,Pennisi Ivana¹,Ahu Prah Diana³⁴,Amoah Linda Eva³⁵,Awandare Gordon³,Hafalla Julius Clemence R.⁴⁵^ORCID,Cunnington Aubrey¹^ORCID,Baum Jake⁶⁷,Rodriguez-Manzano Jesus¹^ORCID,Georgiou Pantelis²

Affiliation:

1. Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, United Kingdom

2. Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, United Kingdom

3. West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Ghana

4. Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

5. Immunology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana

6. Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United Kingdom

7. School of Biomedical Sciences, University of New South Wales Sydney, Sydney, Australia

Abstract

The spread of Plasmodium falciparum parasites with Pfhrp2 / 3 gene deletions presents a major threat to reliable point-of-care diagnosis with current rapid diagnostic tests (RDTs). Novel molecular diagnostics based on nucleic acid amplification are needed to address this liability.

Funder

World Bank African Centres of Excellence Grant

Wellcome African Academy of Sciences DELTAS grant

Royal Society University Research Fellowship

Challenge Grant

UKRI | Engineering and Physical Sciences Research Council

Wellcome Trust

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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