Experiences from 4 Years of Organization of an External Quality Assessment for Mycobacterium tuberculosis Whole-Genome Sequencing in the European Union/European Economic Area-Reference-Cited by-同舟云学术

Experiences from 4 Years of Organization of an External Quality Assessment for Mycobacterium tuberculosis Whole-Genome Sequencing in the European Union/European Economic Area

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Anthony R. M.¹^ORCID,Tagliani E.²,Nikolayevskyy V.³,de Zwaan R.¹,Mulder A.¹,Kamst M.¹,Ködmön C.⁴,van der Werf M. J.⁴,Cirillo D.²^ORCID,van Soolingen D.¹,Taylor Lucy,Rathod Priti,Lippert Kathrin,Mathys Vanessa,Soetaert Karine,Rasmussen Erik Michael,Haanperä Marjo,Morel Florence,Merker Matthias,Roycroft Emma,Fitzgibbon Margaret,Cabibbe Andrea Maurizio,Perrin Monique,Vegard Eldholm,Brønstad Ola,Macedo Rita,Herrera-Leon Laura,Samper Sofia,Mansjö Mikael,Groenheit Ramona,

Affiliation:

1. National Tuberculosis Reference Laboratory, Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands

2. Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy

3. Department of Infectious Diseases, Imperial College London, London, United Kingdom

4. European Centre for Disease Prevention and Control, Stockholm, Sweden

Abstract

The wider availability of whole-genome sequencing (WGS) coupled to new developments in bioinformatic tools and databases to interpret Mycobacterium tuberculosis complex WGS data has accelerated the adoption of this method for the routine prediction of antimycobacterial drug resistance and genotyping, thus necessitating the establishment of a comprehensive external quality control system. Here, we report 4 years of development and results from such a panel.

Funder

European Centre for Disease Prevention and Control

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02244-22

Reference27 articles.

1. PhyResSE: a Web Tool Delineating Mycobacterium tuberculosis Antibiotic Resistance and Lineage from Whole-Genome Sequencing Data

2. Role and value of whole genome sequencing in studying tuberculosis transmission

3. Whole-genome sequencing for prediction of Mycobacterium tuberculosis drug susceptibility and resistance: a retrospective cohort study

4. A robust SNP barcode for typing Mycobacterium tuberculosis complex strains

5. WGS more accurately predicts susceptibility of Mycobacterium tuberculosis to first-line drugs than phenotypic testing

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