Small Molecule Inhibition of an Exopolysaccharide Modification Enzyme is a Viable Strategy To Block Pseudomonas aeruginosa Pel Biofilm Formation-Reference-Cited by-同舟云学术

Small Molecule Inhibition of an Exopolysaccharide Modification Enzyme is a Viable Strategy To Block Pseudomonas aeruginosa Pel Biofilm Formation

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Razvi Erum¹²^ORCID,DiFrancesco Benjamin R.³,Wasney Gregory A.¹⁴,Morrison Zachary A.³,Tam John¹,Auger Anick¹⁵,Baker Perrin¹,Alnabelseya Noor¹²,Rich Jacquelyn D.⁶,Sivarajah Piyanka¹,Whitfield Gregory B.¹²,Harrison Joe J.⁶^ORCID,Melnyk Roman A.¹²^ORCID,Nitz Mark³,Howell P. Lynne¹²^ORCID

Affiliation:

1. Program in Molecular Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada

2. Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

3. Department of Chemistry, University of Toronto, Toronto, Ontario, Canada

4. The Structural & Biophysical Core Facility, The Hospital for Sick Children, Toronto, Ontario, Canada

5. SPARC BioCentre, The Hospital for Sick Children, Toronto, Ontario, Canada

6. Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

Abstract

Present in more than 500 diverse Gram-negative and 900 Gram-positive organisms, the Pel polysaccharide is one of the most phylogenetically widespread biofilm matrix determinants found to date. Partial de- N -acetylation of this α-1,4 linked N -acetylgalactosamine polymer by the carbohydrate modification enzyme PelA is required for Pel-dependent biofilm formation in Pseudomonas aeruginosa and Bacillus cereus .

Funder

Gouvernement du Canada | Canadian Institutes of Health Research

UofA | Canadian Glycomics Network

Cystic Fibrosis Canada

Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada

Government of Canada

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00296-23