Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays-Reference-Cited by-同舟云学术

Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays

Published:2021-12-22 Issue:3 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Valcourt Emelissa J.¹²,Manguiat Kathy¹,Robinson Alyssia¹,Lin Yi-Chan³,Abe Kento T.⁴⁵,Mubareka Samira⁶,Shigayeva Altynay⁴,Zhong Zoë⁴,Girardin Roxie C.⁷,DuPuis Alan⁷,Payne Anne⁷,McDonough Kathleen⁷,Wang Zhen⁸,Gasser Romain⁹¹⁰,Laumaea Annemarie⁹¹⁰,Benlarbi Mehdi¹⁰,Richard Jonathan¹⁰,Prévost Jérémie⁹¹⁰,Anand Sai Priya⁸¹⁰,Dimitrova Kristina¹,Phillipson Clark¹,Evans David H.³,McGeer Allison⁴¹¹¹²,Gingras Anne-Claude⁴⁵,Liang Chen⁸,Petric Martin¹³,Sekirov Inna¹³,Morshed Muhammad¹³,Finzi Andrés⁹¹⁰^ORCID,Drebot Michael¹¹⁴,Wood Heidi¹^ORCID

Affiliation:

1. Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

2. Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada

3. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada

4. Department of Microbiology, Mount Sinai Hospital, Sinai Health System, Toronto, Ontario, Canada

5. Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada

6. Department of Laboratory Medicine and Molecular Diagnostics, Division of Microbiology, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada

7. Wadsworth Center, New York State Department of Health, Albany, New York, USA

8. Department of Medicine, McGill University, Montreal, Quebec, Canada

9. Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Quebec, Canada

10. Centre de Recherche du CHUM, Montreal, Quebec, Canada

11. Institute of Health Policy, Management and Evaluation, University of Toronto, Toronto, Ontario, Canada

12. Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

13. British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, British Columbia, Canada

14. Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

Abstract

SARS-CoV-2, the causal agent of COVID-19, has infected over 150 million people and led to 3.8 million deaths worldwide. With the approval of several efficacious COVID-19 vaccines, methods to evaluate protective immune responses will be crucial for the understanding of long-term immunity in the rapidly growing vaccinated population.

Funder

Public Health Agency of Canada

Fondation du CHUM

Gouvernement du Canada | Canadian Institutes of Health Research

Canada Foundation for Innovation

Ministère de l'Économie, de la Science et de l'Innovation - Québec

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/Spectrum.00886-21