A prebiotic diet modulates the oral microbiome composition and results in the attenuation of oropharyngeal candidiasis in mice
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Published:2023-10-17
Issue:5
Volume:11
Page:
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ISSN:2165-0497
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Container-title:Microbiology Spectrum
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language:en
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Short-container-title:Microbiol Spectr
Author:
Vazquez-Munoz Roberto1ORCID,
Thompson Angela1,
Sobue Takanori1,
Dongari-Bagtzoglou Anna1
Affiliation:
1. Department of General Dentistry, The University of Connecticut Health Center , Farmington, Connecticut, USA
Abstract
ABSTRACT
Oral bacteria can influence the ability of
Candida albicans
to cause oropharyngeal candidiasis (OPC). We recently reported that a
Lactobacillus johnsonii
-enriched oral microbiota reduced
C. albicans
virulence in an immunosuppressed OPC mouse model. As a follow-up, in this work, we aimed to enrich the resident oral
Lactobacillus
communities with a prebiotic diet to further assess their effect on the severity of OPC. We tested the effect of a prebiotic xylo-oligosaccharides (XOS)-enriched diet in the oral global bacterial composition and severity of OPC. We assessed changes in the oral microbiome composition via 16S-rRNA gene high-throughput sequencing, validated by qPCR. The impact of the prebiotic diet on
Candida
infection was assessed by quantifying changes in oral fungal and bacterial biomass and scoring tongue lesions. Contrary to expectations, oral
Lactobacillus
communities were not enriched by the XOS-supplemented diet. Yet, XOS modulated the oral microbiome composition, increasing
Bifidobacterium
abundance and reducing enterococci and staphylococci. In the OPC model, the XOS diet attenuated
Candida
virulence and bacterial dysbiosis, increasing lactobacilli and reducing enterococci on the oral mucosa. We conclude that XOS attenuates
Candida
virulence by promoting a bacterial microbiome structure more resilient to
Candida
infection.
IMPORTANCE
This is the first study on the effects of a prebiotic diet on the oral mucosal bacterial microbiome and an oropharyngeal candidiasis (OPC) mouse model. We found that xylo-oligosaccharides change the oral bacterial community composition and attenuate OPC. Our results contribute to the understanding of the impact of the oral bacterial communities on
Candida
virulence.
Funder
HHS | NIH | National Institute of Dental and Craniofacial Research
HHS | NIH | National Institute of General Medical Sciences
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology
Cited by
1 articles.
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