The Effect of Inoculum Size on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis-Reference-Cited by-同舟云学术

The Effect of Inoculum Size on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Yildirim Kubra¹²^ORCID,Atas Cemilenur³^ORCID,Simsek Ece¹²³^ORCID,Coban Ahmet Yilmaz¹²³^ORCID

Affiliation:

1. Akdeniz University Tuberculosis Research Center, Antalya, Turkey

2. Akdeniz University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Antalya, Turkey

3. Akdeniz University Institute of Health Sciences, Department of Medical Biotechnology, Antalya, Turkey

Abstract

During DST application, it can be challenging to evenly homogenize TB cell clumps with lipid-rich cell walls. These experiments must be carried out under Biosafety Level-3 (BSL-3) laboratory conditions with personal protective equipment and taking safety precautions because the procedures applied at this stage cause the formation of bacillus-laden aerosols and carry a serious risk of transmission.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00319-23

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4. Antimicrobial susceptibility testing of Mycobacterium tuberculosis complex isolates – the EUCAST broth microdilution reference method for MIC determination

5. Clinical and Laboratory Standards Institute. 2018. Susceptibility testing of Mycobacteria Nocardia spp., and other aerobic actinomycetes, 3rd ed. CLSI standard M24. CLSI, Wayne, PA.

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