Engineered Fluorescent Strains of Cryptococcus neoformans: a Versatile Toolbox for Studies of Host-Pathogen Interactions and Fungal Biology, Including the Viable but Nonculturable State

Author:

de Castro Raffael Júnio Araújo12ORCID,Rêgo Marco Túlio Aidar Mariano1,Brandão Fabiana S.3,Pérez Ana Laura Alfonso4,De Marco Janice Lisboa4,Poças-Fonseca Marcio José5ORCID,Nichols Connie6,Alspaugh J. Andrew6ORCID,Felipe Maria Sueli S.7,Alanio Alexandre28ORCID,Bocca Anamélia Lorenzetti1ORCID,Fernandes Larissa19ORCID

Affiliation:

1. Laboratory of Applied Immunology, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Brasília, Federal District, Brazil

2. CNRS, Unité de Mycologie Moléculaire, Centre National de Référence Mycoses et Antifongiques, Institut Pasteur, Paris, France

3. Faculty of Health Science, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Brasília, Federal District, Brazil

4. Department of Cell Biology, Institute of Biological Sciences, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Brasilia, Federal District, Brazil

5. Department of Genetics and Morphology, Institute of Biological Sciences, Campus Darcy Ribeiro, University of Brasília, Asa Norte, Brasília, Federal District, Brazil

6. Duke University School of Medicine, Department of Medicine, Durham, North Carolina, USA

7. Catholic University of Brasilia, Campus Asa Norte, Asa Norte, Brasília, Federal District, Brazil

8. Laboratoire de Mycologie et Parasitologie, AP-HP, Hôpital Saint Louis, Université Paris Diderot, Sorbonne Paris Cité, Paris, France

9. Faculty of Ceilândia, Campus UnB Ceilândia, University of Brasília, Ceilândia Sul, Brasília, Federal District, Brazil

Abstract

Cryptococcus neoformans is a human-pathogenic yeast that can undergo a dormant state and is responsible for over 180,000 deaths annually worldwide. We engineered a set of fluorescent transformants to aid in research on C. neoformans .

Funder

MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação de Apoio à Pesquisa do Distrito Federal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Agence Nationale de la Recherche

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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