Establishment of Continuous In Vitro Culture of Babesia gibsoni by Using VP-SFM Medium with Low-Concentration Serum

Author:

Li Dongfang12,Wang Lingna12,Guan Xingai12,Wang Sen12,Liu Qin12,Chen Fangwei12,Zheng Yaxin12,He Lan12,Zhao Junlong12

Affiliation:

1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China

2. Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, China

Abstract

The VP-SFM as a base medium supplemented with AlbuMAX I and a low concentration of canine serum (2.5%) allowed the continuous in vitro culture of Babesia gibsoni at both small and large volumes, which was to meet different experimental needs, such as long-term culture and obtaining high parasitemia and subclone culture. The establishment of in vitro culture systems allows researchers to better understand the metabolism and growth patterns of Babesia .

Funder

MOST | National Key Research and Development Program of China

National Natural Science Foundation of China

Top-notch Young Talent Supporting Program

CSU | Fundamental Research Funds for Central Universities of the Central South University

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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