Application of Metagenomic Next-Generation Sequencing (mNGS) Using Bronchoalveolar Lavage Fluid (BALF) in Diagnosing Pneumonia of Children-Reference-Cited by-同舟云学术

Application of Metagenomic Next-Generation Sequencing (mNGS) Using Bronchoalveolar Lavage Fluid (BALF) in Diagnosing Pneumonia of Children

Published:2022-10-26 Issue:5 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Yang Aimei¹,Chen Chen²,Hu Yan¹,Zheng Guilang¹,Chen Peiling¹,Xie Zhiwei²,Fan Huifeng²,Sun Yueyu¹,Wu Peiqiong²,Jiang Wenhui²,Wang Chun¹,Zhang Jingwen¹,Zhang Dongwei²,Wang Jing¹,Hu Xiaoyin²,Xia Han³,Yin Genquan²,Guo Yuxiong¹

Affiliation:

1. Department of Pediatric Intensive Care Unit, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China

2. Pediatric Respiratory Department, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China

3. Department of Scientific Affairs, Hugobiotech Co., Ltd., Beijing, China

Abstract

Our study indicates high efficiency of mNGS using BALF for the detection of causative pathogens that cause pneumonia in children. mNGS can be a potential diagnostic tool to supplement conventional methods for children’s pneumonia.

Funder

The Science and Technology Project of Xi’an

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01488-22

Reference38 articles.

1. World Health Organization. 2020. Global health estimates: life expectancy and leading causes of death and disability. WHO Geneva Switzerland. https://www.who.int/data/gho/data/themes/mortality-and-global-health-estimates.

2. Global, regional, and national causes of under-5 mortality in 2000–19: an updated systematic analysis with implications for the Sustainable Development Goals

3. Microbial aetiology of community-acquired pneumonia and its relation to severity

4. International ERS/ESICM/ESCMID/ALAT guidelines for the management of hospital-acquired pneumonia and ventilator-associated pneumonia

5. Pneumonia in Hospitalized Children

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