Hypervariable-Locus Melting Typing: a Novel Approach for More Effective High-Resolution Melting-Based Typing, Suitable for Large Microbiological Surveillance Programs-Reference-Cited by-同舟云学术

Hypervariable-Locus Melting Typing: a Novel Approach for More Effective High-Resolution Melting-Based Typing, Suitable for Large Microbiological Surveillance Programs

Published:2022-08-31 Issue:4 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Perini Matteo¹^ORCID,Piazza Aurora²^ORCID,Panelli Simona¹,Papaleo Stella¹,Alvaro Alessandro¹,Vailati Francesca³,Corbella Marta⁴,Saluzzo Francesca⁵^ORCID,Gona Floriana⁶,Castelli Daniele⁷,Farina Claudio³,Marone Piero⁴,Cirillo Daniela Maria⁵^ORCID,Cavallero Annalisa⁷,Zuccotti Gian Vincenzo¹⁸,Comandatore Francesco¹^ORCID

Affiliation:

1. Department of Biomedical and Clinical Sciences, Romeo and Enrica Invernizzi Pediatric Clinical Research Center, Università Di Milano, Milan, Italy

2. Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy

3. Microbiology Institute, ASST Papa Giovanni XXIII, Bergamo, Italy

4. Microbiology and Virology Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy

5. Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy

6. Laboratory Microbiology and Virology, Ospedale San Raffaele Dibit, Milan, Italy

7. Laboratory of Microbiology, ASST Monza, San Gerardo Hospital, Monza, Italy

8. Department of Pediatrics, Children’s Hospital Vittore Buzzi, Università Di Milano, Milan, Italy

Abstract

In this work, we describe hypervariable-locus melting typing (HLMT), a novel fast approach to pathogen typing using the high-resolution melting (HRM) assay. The method includes a novel approach for gene target selection, primer design, and HRM data analysis.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01009-22

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